Variants of Alpha-Fetoprotein Coding and Expression Sequences

Hiroshi Kubota (Inventor), Lola M. Reid (Inventor), Robert Storms (Inventor)

Research output: Patent

Abstract

The invention discloses the sequences of variant forms of alpha-fetoprotein transcripts that have been identified in human hemopoietic progenitors but not in differentiated mature cells. The variant forms of AFP (vAFP) cDNA sequences isolated from a multipotent hemopoietic cell line, K562, differ from the authentic AFP transcript, consisting of 15 exons, by lacking only exon 1. Instead of exon 1, vAFP transcripts use an additional one or two exons located in the 5'-untranslated region of the AFP gene. K562 expressed selectively vAFP, whereas a hepatocellular carcinoma cell line, HepG2, showed no detectable expression of vAFP. In normal adult tissues, vAFP transcripts is detected in the bone marrow, thymus and brain, but not the spleen, suggesting the expression occurs in normal hemopoietic progenitors. Moreover, CD34+Lin- hemopoietic stem/progenitor cells purified by flow cytometric sorting also express the variant transcripts.
LanguageEnglish
Patent number8137914
IPCC12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101)
StatePublished - 1800

Fingerprint

alpha-Fetoproteins
Exons
Stem Cells
Cell Line
5' Untranslated Regions
Thymus Gland
Hepatocellular Carcinoma
Spleen
Complementary DNA
Bone Marrow
Brain
Genes

Cite this

Kubota, H., Reid, L. M., & Storms, R. (1800). IPC No. C12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101). Variants of Alpha-Fetoprotein Coding and Expression Sequences (Patent No. 8137914.)

Variants of Alpha-Fetoprotein Coding and Expression Sequences. / Kubota, Hiroshi (Inventor); Reid, Lola M. (Inventor); Storms, Robert (Inventor).

IPC No.: C12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101). Patent No.: 8137914.

Research output: Patent

Kubota, H, Reid, LM & Storms, R 1800, Variants of Alpha-Fetoprotein Coding and Expression Sequences, Patent No. 8137914, IPC No. C12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101).
Kubota H, Reid LM, Storms R, inventors. Variants of Alpha-Fetoprotein Coding and Expression Sequences. C12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101). 1800
Kubota, Hiroshi (Inventor) ; Reid, Lola M. (Inventor) ; Storms, Robert (Inventor). / Variants of Alpha-Fetoprotein Coding and Expression Sequences. IPC No.: C12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101). Patent No.: 8137914.
@misc{cf1b18e1b5624160ade25f024ba438bd,
title = "Variants of Alpha-Fetoprotein Coding and Expression Sequences",
abstract = "The invention discloses the sequences of variant forms of alpha-fetoprotein transcripts that have been identified in human hemopoietic progenitors but not in differentiated mature cells. The variant forms of AFP (vAFP) cDNA sequences isolated from a multipotent hemopoietic cell line, K562, differ from the authentic AFP transcript, consisting of 15 exons, by lacking only exon 1. Instead of exon 1, vAFP transcripts use an additional one or two exons located in the 5'-untranslated region of the AFP gene. K562 expressed selectively vAFP, whereas a hepatocellular carcinoma cell line, HepG2, showed no detectable expression of vAFP. In normal adult tissues, vAFP transcripts is detected in the bone marrow, thymus and brain, but not the spleen, suggesting the expression occurs in normal hemopoietic progenitors. Moreover, CD34+Lin- hemopoietic stem/progenitor cells purified by flow cytometric sorting also express the variant transcripts.",
author = "Hiroshi Kubota and Reid, {Lola M.} and Robert Storms",
note = "Status: published applicationnumber: 11/963,207 usclass: 435/6.11 ; 435/6.12; 435/91.2; 536/24.31; 536/24.33 applicationnumber: 11/963,207; 8137914; C12Q 1/68 (20060101); C12N 15/11 (20060101); C12P 19/34 (20060101)",
year = "1800",
language = "English",
type = "Patent",

}

TY - PAT

T1 - Variants of Alpha-Fetoprotein Coding and Expression Sequences

AU - Kubota,Hiroshi

AU - Reid,Lola M.

AU - Storms,Robert

N1 - Status: published applicationnumber: 11/963,207 usclass: 435/6.11 ; 435/6.12; 435/91.2; 536/24.31; 536/24.33 applicationnumber: 11/963,207

PY - 1800

Y1 - 1800

N2 - The invention discloses the sequences of variant forms of alpha-fetoprotein transcripts that have been identified in human hemopoietic progenitors but not in differentiated mature cells. The variant forms of AFP (vAFP) cDNA sequences isolated from a multipotent hemopoietic cell line, K562, differ from the authentic AFP transcript, consisting of 15 exons, by lacking only exon 1. Instead of exon 1, vAFP transcripts use an additional one or two exons located in the 5'-untranslated region of the AFP gene. K562 expressed selectively vAFP, whereas a hepatocellular carcinoma cell line, HepG2, showed no detectable expression of vAFP. In normal adult tissues, vAFP transcripts is detected in the bone marrow, thymus and brain, but not the spleen, suggesting the expression occurs in normal hemopoietic progenitors. Moreover, CD34+Lin- hemopoietic stem/progenitor cells purified by flow cytometric sorting also express the variant transcripts.

AB - The invention discloses the sequences of variant forms of alpha-fetoprotein transcripts that have been identified in human hemopoietic progenitors but not in differentiated mature cells. The variant forms of AFP (vAFP) cDNA sequences isolated from a multipotent hemopoietic cell line, K562, differ from the authentic AFP transcript, consisting of 15 exons, by lacking only exon 1. Instead of exon 1, vAFP transcripts use an additional one or two exons located in the 5'-untranslated region of the AFP gene. K562 expressed selectively vAFP, whereas a hepatocellular carcinoma cell line, HepG2, showed no detectable expression of vAFP. In normal adult tissues, vAFP transcripts is detected in the bone marrow, thymus and brain, but not the spleen, suggesting the expression occurs in normal hemopoietic progenitors. Moreover, CD34+Lin- hemopoietic stem/progenitor cells purified by flow cytometric sorting also express the variant transcripts.

M3 - Patent

M1 - 8137914

ER -