pH-Sensitive Immunoliposomes

Leaf Huang, Jerome Connor, Chen Yen Wang

Research output: Research - peer-reviewArticle

  • 22 Citations

Abstract

This chapter discusses the use of pH sensitive immunoliposomes. In an effort to design immunoliposomes that can also become fusion active when exposed to a mildly acidic environment, one have developed the pH-sensitive immunoliposomes. The design of the antibody-free, pH sensitive liposomes has been described in detail. Preparation of the pH-sensitive immunoliposomes is described in two steps in this chapter. The procedures for fatty acylation of antibody are discussed first. Incorporation of the fatty acyl antibody into the pH-sensitive liposomes is then described. Characterization of the pH-sensitive immunoliposomes and their interaction with target cells is also presented. In this case, liposome contents are delivered to the lysosomes of the target cells. Whether the contents are degraded or inactivated by the lysosomal hydrolases and whether the contents are released intact from the lysosomes, depends on the nature of the entrapped molecule. In any event, cytoplasmic delivery by pH-sensitive immunoliposomes has greatly increased their carder potential.

LanguageEnglish (US)
Pages88-99
Number of pages12
JournalMethods in Enzymology
Volume149
Issue numberC
DOIs
StatePublished - Jan 1 1987

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L Cells (Cell Line)
Drug Carriers
Acylation
Liposomes
Fluorescent Antibody Technique
Fatty Acids
Antibodies
Delta wing aircraft
Lysosomes
Hydrolases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Huang, L., Connor, J., & Wang, C. Y. (1987). pH-Sensitive Immunoliposomes. Methods in Enzymology, 149(C), 88-99. DOI: 10.1016/0076-6879(87)49046-0

pH-Sensitive Immunoliposomes. / Huang, Leaf; Connor, Jerome; Wang, Chen Yen.

In: Methods in Enzymology, Vol. 149, No. C, 01.01.1987, p. 88-99.

Research output: Research - peer-reviewArticle

Huang, L, Connor, J & Wang, CY 1987, 'pH-Sensitive Immunoliposomes' Methods in Enzymology, vol 149, no. C, pp. 88-99. DOI: 10.1016/0076-6879(87)49046-0
Huang L, Connor J, Wang CY. pH-Sensitive Immunoliposomes. Methods in Enzymology. 1987 Jan 1;149(C):88-99. Available from, DOI: 10.1016/0076-6879(87)49046-0
Huang, Leaf ; Connor, Jerome ; Wang, Chen Yen. / pH-Sensitive Immunoliposomes. In: Methods in Enzymology. 1987 ; Vol. 149, No. C. pp. 88-99
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