Effects of sample handling and analytical procedures on thyroid hormone concentrations in pregnant women's plasma

Gro Dehli Villanger, Emily Learner, Matthew P. Longnecker, Helga Ask, Heidi Aase, R. Thomas Zoeller, Gun P. Knudsen, Ted Reichborn-Kjennerud, Pål Zeiner, Stephanie M. Engel

Research output: Research - peer-reviewArticle

Abstract

Background: Maternal thyroid function is a critical mediator of fetal brain development. Pregnancy-related physiologic changes and handling conditions of blood samples may influence thyroid hormone biomarkers. We investigated the reliability of thyroid hormone biomarkers in plasma of pregnant women under various handling conditions. Methods: We enrolled 17 pregnant women; collected serum and plasma were immediately frozen. Additional plasma aliquots were subjected to different handling conditions before the analysis of thyroid biomarkers: storage at room temperature for 24 or 48 hours before freezing and an extra freeze-Thaw cycle. We estimated free thyroid hormone indices in plasma based on T3 uptake. Results: High correlations between plasma and serum (>0.94) and intraclass correlation coefficients for plasma handling conditions (0.96 to 1.00) indicated excellent reliability for all thyroid hormone biomarkers. Conclusion: Delayed freezing and freeze-Thaw cycles did not affect reliability of biomarkers of thyroid function in plasma during pregnancy. See video abstract at, http://links.lww.com/EDE/B180.

LanguageEnglish (US)
Pages365-369
Number of pages5
JournalEpidemiology
Volume28
Issue number3
DOIs
StatePublished - May 1 2017

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Thyroid Hormones
Pregnant Women
Biomarkers
Thyroid Gland
Freezing
Pregnancy
Serum
Fetal Development
Mothers
Temperature
Brain

ASJC Scopus subject areas

  • Epidemiology

Cite this

Villanger, G. D., Learner, E., Longnecker, M. P., Ask, H., Aase, H., Zoeller, R. T., ... Engel, S. M. (2017). Effects of sample handling and analytical procedures on thyroid hormone concentrations in pregnant women's plasma. Epidemiology, 28(3), 365-369. DOI: 10.1097/EDE.0000000000000606

Effects of sample handling and analytical procedures on thyroid hormone concentrations in pregnant women's plasma. / Villanger, Gro Dehli; Learner, Emily; Longnecker, Matthew P.; Ask, Helga; Aase, Heidi; Zoeller, R. Thomas; Knudsen, Gun P.; Reichborn-Kjennerud, Ted; Zeiner, Pål; Engel, Stephanie M.

In: Epidemiology, Vol. 28, No. 3, 01.05.2017, p. 365-369.

Research output: Research - peer-reviewArticle

Villanger, GD, Learner, E, Longnecker, MP, Ask, H, Aase, H, Zoeller, RT, Knudsen, GP, Reichborn-Kjennerud, T, Zeiner, P & Engel, SM 2017, 'Effects of sample handling and analytical procedures on thyroid hormone concentrations in pregnant women's plasma' Epidemiology, vol 28, no. 3, pp. 365-369. DOI: 10.1097/EDE.0000000000000606
Villanger GD, Learner E, Longnecker MP, Ask H, Aase H, Zoeller RT et al. Effects of sample handling and analytical procedures on thyroid hormone concentrations in pregnant women's plasma. Epidemiology. 2017 May 1;28(3):365-369. Available from, DOI: 10.1097/EDE.0000000000000606
Villanger, Gro Dehli ; Learner, Emily ; Longnecker, Matthew P. ; Ask, Helga ; Aase, Heidi ; Zoeller, R. Thomas ; Knudsen, Gun P. ; Reichborn-Kjennerud, Ted ; Zeiner, Pål ; Engel, Stephanie M./ Effects of sample handling and analytical procedures on thyroid hormone concentrations in pregnant women's plasma. In: Epidemiology. 2017 ; Vol. 28, No. 3. pp. 365-369
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