Diagnosing acute HIV infection: The performance of quantitative HIV-1 RNA testing (viral load) in the 2014 laboratory testing algorithm

Hsiu Wu, Stephanie E. Cohen, Emily Westheimer, Cynthia L. Gay, Laura Hall, Charles Rose, Lisa B. Hightow-Weidman, Severin Gose, Jie Fu, Philip J. Peters

Research output: Contribution to journalArticle

Abstract

New recommendations for laboratory diagnosis of HIV infection in the United States were published in 2014. The updated testing algorithm includes a qualitative HIV-1 RNA assay to resolve discordant immunoassay results and to identify acute HIV-1 infection (AHI). The qualitative HIV-1 RNA assay is not widely available; therefore, we evaluated the performance of a more widely available quantitative HIV-1 RNA assay, viral load, for diagnosing AHI. We determined that quantitative viral loads consistently distinguished AHI from a false-positive immunoassay result. Among 100 study participants with AHI and a viral load result, the estimated geometric mean viral load was 1,377,793 copies/mL.

LanguageEnglish (US)
Pages85-86
Number of pages2
JournalJournal of Clinical Virology
Volume93
DOIs
StatePublished - Aug 1 2017

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Viral Load
HIV Infections
HIV-1
RNA
Immunoassay
Clinical Laboratory Techniques

Keywords

  • Acute HIV infection
  • Fourth-generation immunoassay
  • HIV diagnosis
  • HIV testing
  • Quantitative HIV-1 viral load

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Diagnosing acute HIV infection : The performance of quantitative HIV-1 RNA testing (viral load) in the 2014 laboratory testing algorithm. / Wu, Hsiu; Cohen, Stephanie E.; Westheimer, Emily; Gay, Cynthia L.; Hall, Laura; Rose, Charles; Hightow-Weidman, Lisa B.; Gose, Severin; Fu, Jie; Peters, Philip J.

In: Journal of Clinical Virology, Vol. 93, 01.08.2017, p. 85-86.

Research output: Contribution to journalArticle

Wu, Hsiu ; Cohen, Stephanie E. ; Westheimer, Emily ; Gay, Cynthia L. ; Hall, Laura ; Rose, Charles ; Hightow-Weidman, Lisa B. ; Gose, Severin ; Fu, Jie ; Peters, Philip J./ Diagnosing acute HIV infection : The performance of quantitative HIV-1 RNA testing (viral load) in the 2014 laboratory testing algorithm. In: Journal of Clinical Virology. 2017 ; Vol. 93. pp. 85-86
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