Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body

Michele Alves-Bezerra, Isabela B. Ramos, Iron F. De Paula, Clarissa M. Maya-Monteiro, Eric L. Klett, Rosalind A. Coleman, Katia C. Gondim

Research output: Research - peer-reviewArticle

Abstract

Glycerol-3-phosphate acyltransferases (GPAT) catalyze the initial and rate-limiting step for the de novo synthesis of triacylglycerol (TAG). Four mammalian GPAT isoforms have been identified: the mitochondria-associated GPAT1 and 2, and the endoplasmic reticulum (ER)-associated GPAT3 and 4. In the insect Rhodnius prolixus, a vector of Chagas’ disease, we previously predicted a mitochondrial-like isoform (RhoprGPAT1) from genomic data. In the current study, we clone the RhoprGPAT1 coding sequence and identify an ER-associated GPAT (RhoprGPAT4) as the second isoform in the insect. RhoprGPAT1 contributes 15% of the total GPAT activity in anterior midgut, 50% in posterior midgut and fat body, and 70% in the ovary. The RhoprGpat1 gene is the predominant transcript in the midgut and fat body. To evaluate the physiological relevance of RhoprGPAT1, we generate RhoprGPAT1-deficient insects. The knockdown of RhoprGpat1 results in 50% and 65% decrease in TAG content in the posterior midgut and fat body, respectively. RhoprGpat1-deficient insects also exhibits impaired lipid droplet expansion and a 2-fold increase in fatty acid β-oxidation rates in the fat body. We propose that the RhoprGPAT1 mitochondrial-like isoform is required to channel fatty acyl chains towards TAG synthesis and away from β-oxidation. Such a process is crucial for the insect lipid homeostasis.

LanguageEnglish (US)
Pages324-336
Number of pages13
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1862
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Acyltransferases
Fat Body
Insects
Triglycerides
Fatty Acids
alpha-glycerophosphoric acid
Protein Isoforms
Endoplasmic Reticulum
Rhodnius
Chagas Disease
Ovary
Mitochondria
Homeostasis
Clone Cells
Lipids
Genes
Lipid Droplets

Keywords

  • Beta-oxidation
  • Fatty acid metabolism
  • Fatty acid oxidation
  • Insect physiology
  • Lipids
  • Triacylglycerol synthesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body. / Alves-Bezerra, Michele; Ramos, Isabela B.; De Paula, Iron F.; Maya-Monteiro, Clarissa M.; Klett, Eric L.; Coleman, Rosalind A.; Gondim, Katia C.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1862, No. 3, 01.03.2017, p. 324-336.

Research output: Research - peer-reviewArticle

Alves-Bezerra, Michele ; Ramos, Isabela B. ; De Paula, Iron F. ; Maya-Monteiro, Clarissa M. ; Klett, Eric L. ; Coleman, Rosalind A. ; Gondim, Katia C./ Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2017 ; Vol. 1862, No. 3. pp. 324-336
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