Coordinating cell cycle-regulated histone gene expression through assembly and function of the Histone Locus Body

Robert J. Duronio, William F. Marzluff

Research output: Contribution to journalReview article

  • 10 Citations

Abstract

Metazoan replication-dependent (RD) histone genes encode the only known cellular mRNAs that are not polyadenylated. These mRNAs end instead in a conserved stem-loop, which is formed by an endonucleolytic cleavage of the pre-mRNA. The genes for all 5 histone proteins are clustered in all metazoans and coordinately regulated with high levels of expression during S phase. Production of histone mRNAs occurs in a nuclear body called the Histone Locus Body (HLB), a subdomain of the nucleus defined by a concentration of factors necessary for histone gene transcription and pre-mRNA processing. These factors include the scaffolding protein NPAT, essential for histone gene transcription, and FLASH and U7 snRNP, both essential for histone pre-mRNA processing. Histone gene expression is activated by Cyclin E/Cdk2-mediated phosphorylation of NPAT at the G1-S transition. The concentration of factors within the HLB couples transcription with pre-mRNA processing, enhancing the efficiency of histone mRNA biosynthesis.

LanguageEnglish (US)
Pages726-738
Number of pages13
JournalRNA biology
Volume14
Issue number6
DOIs
StatePublished - Jun 3 2017

Fingerprint

Histones
Cell Cycle
Gene Expression
RNA Precursors
Messenger RNA
U7 Small Nuclear Ribonucleoproteins
Genes
Cyclin E
Essential Genes
S Phase
Proteins
Phosphorylation

Keywords

  • Cell cycle
  • Drosophila
  • histone genes
  • mRNA processing
  • nuclear body

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Coordinating cell cycle-regulated histone gene expression through assembly and function of the Histone Locus Body. / Duronio, Robert J.; Marzluff, William F.

In: RNA biology, Vol. 14, No. 6, 03.06.2017, p. 726-738.

Research output: Contribution to journalReview article

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