Cell cycle proliferation decisions: the impact of single cell analyses

Jacob P. Matson, Jeanette G. Cook

Research output: Contribution to journalReview article

  • 3 Citations

Abstract

Cell proliferation is a fundamental requirement for organismal development and homeostasis. The mammalian cell division cycle is tightly controlled to ensure complete and precise genome duplication and segregation of replicated chromosomes to daughter cells. The onset of DNA replication marks an irreversible commitment to cell division, and the accumulated efforts of many decades of molecular and cellular studies have probed this cellular decision, commonly called the restriction point. Despite a long-standing conceptual framework of the restriction point for progression through G1 phase into S phase or exit from G1 phase to quiescence (G0), recent technical advances in quantitative single cell analysis of mammalian cells have provided new insights. Significant intercellular heterogeneity revealed by single cell studies and the discovery of discrete subpopulations in proliferating cultures suggests the need for an even more nuanced understanding of cell proliferation decisions. In this review, we describe some of the recent developments in the cell cycle field made possible by quantitative single cell experimental approaches.

LanguageEnglish (US)
Pages362-375
Number of pages14
JournalFEBS Journal
Volume284
Issue number3
DOIs
StatePublished - Feb 1 2017

Fingerprint

Single-Cell Analysis
Cell Cycle
Cells
Cell Proliferation
Cell proliferation
G1 Phase
Chromosome Segregation
Chromosomes
DNA Replication
S Phase
Cell Division
Homeostasis
Genes
Genome
DNA

Keywords

  • biosensor
  • cell cycle
  • cell division
  • G0
  • G1
  • G2
  • quiescence
  • restriction point
  • review
  • single cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cell cycle proliferation decisions : the impact of single cell analyses. / Matson, Jacob P.; Cook, Jeanette G.

In: FEBS Journal, Vol. 284, No. 3, 01.02.2017, p. 362-375.

Research output: Contribution to journalReview article

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