Beyond attachment: Roles of DC-SIGN in dengue virus infection

Ping Liu, Marc Ridilla, Pratik Patel, Laurie Betts, Emily Gallichotte, Lidea Shahidi, Nancy L. Thompson, Ken Jacobson

Research output: Research - peer-reviewArticle

  • 1 Citations

Abstract

Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), a C-type lectin expressed on the plasma membrane by human immature dendritic cells, is a receptor for numerous viruses including Ebola, SARS and dengue. A controversial question has been whether DC-SIGN functions as a complete receptor for both binding and internalization of dengue virus (DENV) or whether it is solely a cell surface attachment factor, requiring either hand-off to another receptor or a co-receptor for internalization. To examine this question, we used 4 cell types: human immature dendritic cells and NIH3T3 cells expressing either wild-type DC-SIGN or 2 internalization-deficient DC-SIGN mutants, in which either the 3 cytoplasmic internalization motifs are silenced by alanine substitutions or the cytoplasmic region is truncated. Using confocal and super-resolution imaging and high content single particle tracking, we investigated DENV binding, DC-SIGN surface transport, endocytosis, as well as cell infectivity. DC-SIGN was found colocalized with DENV inside cells suggesting hand-off at the plasma membrane to another receptor did not occur. Moreover, all 3 DC-SIGN molecules on NIH3T3 cells supported cell infection. These results imply the involvement of a co-receptor because cells expressing the internalization-deficient mutants could still be infected.

LanguageEnglish (US)
Pages218-231
Number of pages14
JournalTraffic
Volume18
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Dengue Virus
Cell Adhesion Molecules
Virus Diseases
Dendritic Cells
Viruses
Hand
Cell Membrane
Cell membranes
Cells
Ebolavirus
C-Type Lectins
Virus Receptors
Virus Attachment
Endocytosis
Alanine
Infection
Substitution reactions
Imaging techniques
Molecules

Keywords

  • antigen-presenting cells
  • C-type lectin receptor
  • DC-SIGN
  • dengue virus
  • fluorescence microscopy
  • in vivo trafficking
  • quantitative colocalization
  • super-resolution imaging
  • viral entry
  • viral receptor

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Liu, P., Ridilla, M., Patel, P., Betts, L., Gallichotte, E., Shahidi, L., ... Jacobson, K. (2017). Beyond attachment: Roles of DC-SIGN in dengue virus infection. Traffic, 18(4), 218-231. DOI: 10.1111/tra.12469

Beyond attachment : Roles of DC-SIGN in dengue virus infection. / Liu, Ping; Ridilla, Marc; Patel, Pratik; Betts, Laurie; Gallichotte, Emily; Shahidi, Lidea; Thompson, Nancy L.; Jacobson, Ken.

In: Traffic, Vol. 18, No. 4, 01.04.2017, p. 218-231.

Research output: Research - peer-reviewArticle

Liu, P, Ridilla, M, Patel, P, Betts, L, Gallichotte, E, Shahidi, L, Thompson, NL & Jacobson, K 2017, 'Beyond attachment: Roles of DC-SIGN in dengue virus infection' Traffic, vol 18, no. 4, pp. 218-231. DOI: 10.1111/tra.12469
Liu P, Ridilla M, Patel P, Betts L, Gallichotte E, Shahidi L et al. Beyond attachment: Roles of DC-SIGN in dengue virus infection. Traffic. 2017 Apr 1;18(4):218-231. Available from, DOI: 10.1111/tra.12469
Liu, Ping ; Ridilla, Marc ; Patel, Pratik ; Betts, Laurie ; Gallichotte, Emily ; Shahidi, Lidea ; Thompson, Nancy L. ; Jacobson, Ken. / Beyond attachment : Roles of DC-SIGN in dengue virus infection. In: Traffic. 2017 ; Vol. 18, No. 4. pp. 218-231
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