(ArgGlyAsp)n-Albumin conjugates as a model substratum for integrin-mediated cell adhesion

Y. N. Danilov, R. L. Juliano

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Abstract

We have prepared protein-peptide conjugates composed of bovine serum albumin (BSA) derivatized with short peptides containing the ArgGlyAsp (RGD) sequence derived from the adhesion site of fibronectin. The RGD-BSA conjugates were used to coat tissue culture plastic surfaces which then served as substrata in cell adhesion experiments. Our results indicate that the efficiency of adhesion to RGD-BSA-coated surfaces is highly dependent on the valency of the (RGD)n-BSA conjugates. For example, on surfaces with approximately equal amounts of RGD ligand, CHO cells adhered virtually 100% to the (RGD)n-BSA (n=20.8) conjugate and not at all to the (RGD)n-BSA (n=3.5) conjugate. Adhesion on (RGD)n-BSA-coated substrata and on fibronectin- or vitronectin-coated substrata was also exmined in terms of the relationship between cell adhesion and the intermolecular distances of adsorbed proteins. It was observed that for substrata coated with relatively compact, symmetric molecules, such as RGD-BSA or vitronectin, adhesion dropped off sharply as intermolecular distances increased; by contrast, for fibronectin, a large asymmetric molecule, adhesion declined more gradually as intermolecular distances increased. Finally, we have examined the role of different cell-surface receptors in the process of adhesion to RGD-BSA substrata. Interestingly, competition and blocking experiments with antibodies and with soluble competing proteins suggest that it is the vitronectin receptor rather than the fibronectin receptor which mediates adhesion to RGD-BSA.

LanguageEnglish (US)
Pages186-196
Number of pages11
JournalExperimental Cell Research
Volume182
Issue number1
DOIs
StatePublished - Jan 1 1989

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Bovine Serum Albumin
Cell Adhesion
Integrins
Albumins
Fibronectins
Vitronectin
Integrin alphaVbeta3
Integrin alpha5beta1
Peptides
Proteins
CHO Cells
Cell Surface Receptors
Plastics
Ligands
Antibodies

ASJC Scopus subject areas

  • Cell Biology

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(ArgGlyAsp)n-Albumin conjugates as a model substratum for integrin-mediated cell adhesion. / Danilov, Y. N.; Juliano, R. L.

In: Experimental Cell Research, Vol. 182, No. 1, 01.01.1989, p. 186-196.

Research output: Contribution to journalArticle

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