Antimicrobial activity of ozone and NaF-chlorhexidine on early childhood caries

Marcos Ximenes, Mariane Cardoso, Fernando Astorga, Roland Arnold, Luiz André Pimenta, Ricardo de Sousa Viera

Research output: Contribution to journalArticle

Abstract

An early childhood carie (ECC) is an extremely destructive form of tooth decay. The aim of this study was to investigate the action of ozone (O3), and the association of sodium fluoride (NaF) with chlorhexidine (CHX) on bacteria related to ECC. Overnight culture of the bacteria was performed. On exponential phase the suspension was adjusted (101-108 CFU/mL). A drop (10μL) of each concentration of bacteria was applied on sheep blood agar plates and treated with O3 (2, 20, 200, and 2,000 ppm); after 18 hours, recovery analysis of CFU verified the reduction of bacterial activity. For NaF-CHX, sterile 96-well plates were prepared and divided into groups: G1 (150 μL TSB); G2 (20 μL of bacteria + 25 μL CHX + 25 μL NaF); and G3 (150 μL TSB + 20 μL of bacteria + 50 μL water). The plates were verified by analysis of the optical density (0, 12, 14, 16, and 18 hours). The data from O3 test were submitted to ANOVA and Tukey's test (p < 0.05). For the data from NaF-CHX, the ANOVA 2-way and Bonferroni's test (p < 0.05) were used. The number of CFU/mL showed death > 3log10 (99.9%) for all bacteria (ozone ≥ 20ppm), while the combination of NaF-CHX was more effective (p < 0.001) compared to each substance tested alone and the control group. The antimicrobial agents tested were able to inhibit all bacteria tested; O3 seemed to be a good alternative for controlling progression of carious lesions, while the association of NaF-CHX showed to be a good antimicrobial with easy and inexpensive application.

LanguageEnglish (US)
Article numbere2
JournalBrazilian oral research
Volume31
DOIs
StatePublished - Jan 1 2017

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Chlorhexidine
Ozone
Bacteria
Analysis of Variance
Sodium Fluoride
Anti-Infective Agents
Agar
Sheep
Suspensions
Tooth
Control Groups
Water

Keywords

  • Chlorhexidine
  • Dental caries
  • Ozone
  • Sodium fluoride
  • Tooth deciduous

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antimicrobial activity of ozone and NaF-chlorhexidine on early childhood caries. / Ximenes, Marcos; Cardoso, Mariane; Astorga, Fernando; Arnold, Roland; Pimenta, Luiz André; Viera, Ricardo de Sousa.

In: Brazilian oral research, Vol. 31, e2, 01.01.2017.

Research output: Contribution to journalArticle

Ximenes, Marcos ; Cardoso, Mariane ; Astorga, Fernando ; Arnold, Roland ; Pimenta, Luiz André ; Viera, Ricardo de Sousa. / Antimicrobial activity of ozone and NaF-chlorhexidine on early childhood caries. In: Brazilian oral research. 2017 ; Vol. 31.
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abstract = "An early childhood carie (ECC) is an extremely destructive form of tooth decay. The aim of this study was to investigate the action of ozone (O3), and the association of sodium fluoride (NaF) with chlorhexidine (CHX) on bacteria related to ECC. Overnight culture of the bacteria was performed. On exponential phase the suspension was adjusted (101-108 CFU/mL). A drop (10μL) of each concentration of bacteria was applied on sheep blood agar plates and treated with O3 (2, 20, 200, and 2,000 ppm); after 18 hours, recovery analysis of CFU verified the reduction of bacterial activity. For NaF-CHX, sterile 96-well plates were prepared and divided into groups: G1 (150 μL TSB); G2 (20 μL of bacteria + 25 μL CHX + 25 μL NaF); and G3 (150 μL TSB + 20 μL of bacteria + 50 μL water). The plates were verified by analysis of the optical density (0, 12, 14, 16, and 18 hours). The data from O3 test were submitted to ANOVA and Tukey's test (p < 0.05). For the data from NaF-CHX, the ANOVA 2-way and Bonferroni's test (p < 0.05) were used. The number of CFU/mL showed death > 3log10 (99.9{\%}) for all bacteria (ozone ≥ 20ppm), while the combination of NaF-CHX was more effective (p < 0.001) compared to each substance tested alone and the control group. The antimicrobial agents tested were able to inhibit all bacteria tested; O3 seemed to be a good alternative for controlling progression of carious lesions, while the association of NaF-CHX showed to be a good antimicrobial with easy and inexpensive application.",
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