Androgen receptor regulation by histone methyltransferase Suppressor of variegation 3-9 homolog 2 and Melanoma antigen-A11

Emily B. Askew, Suxia Bai, Amanda B. Parris, John T. Minges, Elizabeth M. Wilson

Research output: Contribution to journalArticle

Abstract

Androgen receptor (AR) transcriptional activity depends on interactions between the AR NH2-terminal region and transcriptional coregulators. A yeast two-hybrid screen of a human testis library using predicted α-helical NH2-terminal fragment AR-(370-420) as bait identified suppressor of variegation 3-9 homolog 2 (SUV39H2) histone methyltransferase as an AR interacting protein. SUV39H2 interaction with AR and the AR coregulator, melanoma antigen-A11 (MAGE-A11), was verified in two-hybrid, in vitro glutathione S-transferase affinity matrix and coimmunoprecipitation assays. Fluorescent immunocytochemistry colocalized SUV39H2 and AR in the cytoplasm without androgen, in the nucleus with androgen, and with MAGE-A11 in the nucleus independent of androgen. Chromatin immunoprecipitation using antibodies raised against SUV39H2 demonstrated androgen-dependent recruitment of AR and SUV39H2 to the androgen-responsive upstream enhancer of the prostate-specific antigen gene. SUV39H2 functioned cooperatively with MAGE-A11 to increase androgen-dependent AR transcriptional activity. SUV39H2 histone methyltransferase is an AR coactivator that increases androgen-dependent transcriptional activity through interactions with AR and MAGE-A11.

LanguageEnglish (US)
Pages42-51
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume443
DOIs
StatePublished - Mar 5 2017

Fingerprint

Melanoma-Specific Antigens
Androgen Receptors
Androgens
histone methyltransferase
Receptor-Interacting Protein Serine-Threonine Kinases
Chromatin Immunoprecipitation
Prostate-Specific Antigen
Glutathione Transferase
Yeast
Chromatin
Testis
Assays
Cytoplasm
Genes
Yeasts
Immunohistochemistry

Keywords

  • Androgen receptor
  • Histone methyltransferase
  • MAGE-A11
  • Melanoma antigen-A11
  • SUV39H2

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Androgen receptor regulation by histone methyltransferase Suppressor of variegation 3-9 homolog 2 and Melanoma antigen-A11. / Askew, Emily B.; Bai, Suxia; Parris, Amanda B.; Minges, John T.; Wilson, Elizabeth M.

In: Molecular and Cellular Endocrinology, Vol. 443, 05.03.2017, p. 42-51.

Research output: Contribution to journalArticle

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