Analysis of polycyclic aromatic hydrocarbons and their metabolites by high-pressure liquid chromatography

Dhiren R. Thakker, Haruhiko Yagi, Donald M. Jerina

Research output: Contribution to journalArticle

  • 27 Citations

Abstract

In conclusion, HPLC has proved to be a mild, convenient, and highly efficient method of analysis of polycyclic aromatic hydrocarbons and their oxygenated metabolites. Since some of the metabolites of polycyclic hydrocarbons are highly labile compounds, they are not amenable to the conventional gas chromatographic—mass spectrometric analysis. The HLPC analysis can be performed at ambient temperatures and practically in an anaerobic environment. Moreover, in reverse-phase chromatography, the metabolites are in chemically inert and highly lipophilic medium. HPLC offers a powerful complement to gas chromatography in that polar, and thermally labile metabolites that would have to be derivatized can be handled directly. Additionally, compounds which differ widely in polarity can be directly quantified in a single reverse-phase analysis without derivatization. HPLC has not only helped to elucidate the complex metabolic pathways of polycyclic hydrocarbons, but has also enabled study of the covalent interaction of the reactive metabolites with cellular macromolecules, there contributing to the elucidation of the mechanism of action of these hydrocarbon carcinogens.

LanguageEnglish (US)
Pages279-296
Number of pages18
JournalMethods in enzymology
Volume52
Issue numberC
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

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High pressure liquid chromatography
Cyclic Hydrocarbons
Polycyclic Aromatic Hydrocarbons
Metabolites
High Pressure Liquid Chromatography
Reverse-Phase Chromatography
Hydrocarbons
Metabolic Networks and Pathways
Gas Chromatography
Carcinogens
Gases
Chromatography
Temperature
Macromolecules
Gas chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Analysis of polycyclic aromatic hydrocarbons and their metabolites by high-pressure liquid chromatography. / Thakker, Dhiren R.; Yagi, Haruhiko; Jerina, Donald M.

In: Methods in enzymology, Vol. 52, No. C, 01.01.1978, p. 279-296.

Research output: Contribution to journalArticle

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