Age-related differences in anxiety-like behavior and amygdalar CCL2 responsiveness to stress following alcohol withdrawal in male Wistar rats

Kathryn M. Harper, Darin J. Knapp, Meredith A. Park, George R. Breese

Research output: Research - peer-reviewArticle

Abstract

Rationale: Behavioral and neuroimmune vulnerability to withdrawal from chronic alcohol varies with age. The relation of anxiety-like behavior to amygdalar CCL2 responses following stress after withdrawal from chronic intermittent alcohol (CIA) was investigated in adolescent and adult rats. Methods: Adolescent and adult Wistar rats were exposed to CIA (three 5-day blocks of dietary alcohol separated by 2 days of withdrawal) at concentrations that created similar blood alcohol levels across age. Twenty-four hours into the final withdrawal, half of the rats were exposed to 1 h of restraint stress. Four hours post-stress, rats were used for behavior or tissue assays. Results: Anxiety-like behavior was increased versus controls by CIA in adolescents and by CIA + stress in adults. CCL2 mRNA was increased versus controls by CIA in adolescents and by CIA and CIA + stress in adults. CCL2 co-localization with neuronal marker NeuN was decreased versus controls by CIA in adolescents and by CIA + stress in adults. CCL2 co-localization with astrocytic marker GFAP was decreased versus controls by CIA and CIA + stress in adolescents, but experimental groups did not differ from controls in adults. CCL2 co-localization with microglial marker Iba1 was decreased versus controls by stress alone in adolescents and by CIA + stress in adults. Conclusions: Changes in CCL2 protein might control behavior at either age but are particularly associated with CIA alone in adolescents and with CIA + stress in adults. That the number of CeA neurons expressing CCL2 was altered after CIA and stress is consistent with CCL2 involvement in neural function.

LanguageEnglish (US)
Pages79-88
Number of pages10
JournalPsychopharmacology
Volume234
Issue number1
DOIs
StatePublished - Jan 1 2017

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Wistar Rats
Anxiety
Alcohols
Behavior Control
Neurons
Messenger RNA
Proteins
Blood Alcohol Content

Keywords

  • Adolescent
  • Adult
  • Astrocytes
  • Ethanol
  • Microglia
  • Neurons

ASJC Scopus subject areas

  • Pharmacology

Cite this

Age-related differences in anxiety-like behavior and amygdalar CCL2 responsiveness to stress following alcohol withdrawal in male Wistar rats. / Harper, Kathryn M.; Knapp, Darin J.; Park, Meredith A.; Breese, George R.

In: Psychopharmacology, Vol. 234, No. 1, 01.01.2017, p. 79-88.

Research output: Research - peer-reviewArticle

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