A novel clerodane diterpene from Vitex cofassus

Faradiba Abdul Rasyid, Shuichi Fukuyoshi, Hirokazu Ando, Katsunori Miyake, Toshiyuki Atsumi, Tetsuo Fujie, Yohei Saito, Masuo Goto, Tomohiro Shinya, Masayuki Mikage, Yohei Sasaki, Kyoko Nakagawa-Goto

Research output: Research - peer-reviewArticle

Abstract

New clerodane diterpene, 16-hydroxy-pentandralactone (1) and known diterpene acuminolide (2) were isolated from the methanol extract of Vitex cofassus leaves. The chemical structure and the absolute configuration of 1 were determined by MS, NMR and electron circular dichroism (ECD) experiments. The isolated compounds were evaluated for their antiproliferative activities against a panel of human tumor cell lines, including a multidrug-resistant (MDR) cell line. Both compounds showed potent antiproliferative activities against all the tested cell lines with IC50 values of 5.4-11.4 μM. Their effects on cell viability were also tested using vascular endothelial growth factor (VEGF)-stimulated human umbilical vein endothelial cells (HUVECs). Compound 1 inhibited VEGF-stimulated HUVEC proliferation in a dose-dependent manner. Based on these results, compound 1 could be a candidate for antitumor agent and inhibitor of angiogenesis.

LanguageEnglish (US)
Pages116-120
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume65
Issue number1
DOIs
StatePublished - 2017

Fingerprint

Clerodane Diterpenes
Vitex
Human Umbilical Vein Endothelial Cells
Vascular Endothelial Growth Factor A
Cell Line
Cells
Angiogenesis Inhibitors
Diterpenes
Circular Dichroism
Tumor Cell Line
Antineoplastic Agents
Methanol
Cell Survival
Cell Proliferation
Electrons
Endothelial cells
Cell proliferation
Tumors
Nuclear magnetic resonance
Experiments

Keywords

  • Antiangiogenic activity
  • Antiproliferative activity
  • Clerodane diterpene
  • Vitex cofassus

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Cite this

Rasyid, F. A., Fukuyoshi, S., Ando, H., Miyake, K., Atsumi, T., Fujie, T., ... Nakagawa-Goto, K. (2017). A novel clerodane diterpene from Vitex cofassus. Chemical and Pharmaceutical Bulletin, 65(1), 116-120. DOI: 10.1248/cpb.c16-00775

A novel clerodane diterpene from Vitex cofassus. / Rasyid, Faradiba Abdul; Fukuyoshi, Shuichi; Ando, Hirokazu; Miyake, Katsunori; Atsumi, Toshiyuki; Fujie, Tetsuo; Saito, Yohei; Goto, Masuo; Shinya, Tomohiro; Mikage, Masayuki; Sasaki, Yohei; Nakagawa-Goto, Kyoko.

In: Chemical and Pharmaceutical Bulletin, Vol. 65, No. 1, 2017, p. 116-120.

Research output: Research - peer-reviewArticle

Rasyid, FA, Fukuyoshi, S, Ando, H, Miyake, K, Atsumi, T, Fujie, T, Saito, Y, Goto, M, Shinya, T, Mikage, M, Sasaki, Y & Nakagawa-Goto, K 2017, 'A novel clerodane diterpene from Vitex cofassus' Chemical and Pharmaceutical Bulletin, vol 65, no. 1, pp. 116-120. DOI: 10.1248/cpb.c16-00775
Rasyid FA, Fukuyoshi S, Ando H, Miyake K, Atsumi T, Fujie T et al. A novel clerodane diterpene from Vitex cofassus. Chemical and Pharmaceutical Bulletin. 2017;65(1):116-120. Available from, DOI: 10.1248/cpb.c16-00775
Rasyid, Faradiba Abdul ; Fukuyoshi, Shuichi ; Ando, Hirokazu ; Miyake, Katsunori ; Atsumi, Toshiyuki ; Fujie, Tetsuo ; Saito, Yohei ; Goto, Masuo ; Shinya, Tomohiro ; Mikage, Masayuki ; Sasaki, Yohei ; Nakagawa-Goto, Kyoko. / A novel clerodane diterpene from Vitex cofassus. In: Chemical and Pharmaceutical Bulletin. 2017 ; Vol. 65, No. 1. pp. 116-120
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