A mouse model for chronic pain-induced increase in ethanol consumption

Ryan K. Butler, Darin J. Knapp, Veronica Ulici, Lara Longobardi, Richard F. Loeser, George R. Breese

Research output: Research - peer-reviewArticle

Abstract

Chronic pain conditions are often comorbid with alcohol abuse. "Self-medication" with alcohol introduces a host of problems associated with the abuse of alcohol which over time has the potential of exacerbating the painful condition. Despite the prevalence of chronic pain being associated with alcohol abuse, rodent models which mimic the comorbid conditions are lacking. In this study, we model osteoarthritis (OA) in C57BL/6J mice by surgically destabilizing the medial meniscus (DMM). Sham-operated mice served as controls. Thirteen weeks after surgery, DMM but not sham-operated mice exhibited pronounced incapacitance of the surgically manipulated hind limb compared with the nonsurgically manipulated hind limb. At this time, the mice were exposed to the 2-bottle ethanol choice, beginning with 2.5% with a gradual increasing to 20%. Compared with sham controls, DMM mice consumed more EtOH and preferred EtOH over water at the 20% EtOH concentration. Histological analysis verified that the DMM mice exhibited significant damage to the articular cartilage and osteophyte growth compared with sham controls and these measures of the severity of OA correlated with the amount of ethanol intake. Thus, the combination of the DMMmodel of OA with the enhanced twobottle ethanol choice is a potential preclinical approach in mice by which the basis of the comorbid association of alcohol abuse and chronic pain conditions can be explored.

LanguageEnglish (US)
Pages457-462
Number of pages6
JournalPain
Volume158
Issue number3
DOIs
StatePublished - Jan 1 2017

Fingerprint

Chronic Pain
Ethanol
Tibial Meniscus
Alcoholism
Osteoarthritis
Extremities
Osteophyte
Self Medication
Articular Cartilage
Inbred C57BL Mouse
Rodentia
Alcohols
Water
Growth

Keywords

  • Alcohol consumption
  • Destabilization of the medial meniscus
  • Osteoarthritis

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

A mouse model for chronic pain-induced increase in ethanol consumption. / Butler, Ryan K.; Knapp, Darin J.; Ulici, Veronica; Longobardi, Lara; Loeser, Richard F.; Breese, George R.

In: Pain, Vol. 158, No. 3, 01.01.2017, p. 457-462.

Research output: Research - peer-reviewArticle

Butler, Ryan K. ; Knapp, Darin J. ; Ulici, Veronica ; Longobardi, Lara ; Loeser, Richard F. ; Breese, George R./ A mouse model for chronic pain-induced increase in ethanol consumption. In: Pain. 2017 ; Vol. 158, No. 3. pp. 457-462
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