A method for evaluating the murine pulmonary vasculature using micro-computed tomography

Michael R. Phillips, Scott M. Moore, Mansi Shah, Clara Lee, Yueh Z. Lee, James E. Faber, Sean E. McLean

Research output: Contribution to journalArticle

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Abstract

Background Significant mortality and morbidity are associated with alterations in the pulmonary vasculature. While techniques have been described for quantitative morphometry of whole-lung arterial trees in larger animals, no methods have been described in mice. We report a method for the quantitative assessment of murine pulmonary arterial vasculature using high-resolution computed tomography scanning. Methods Mice were harvested at 2 weeks, 4 weeks, and 3 months of age. The pulmonary artery vascular tree was pressure perfused to maximal dilation with a radio-opaque casting material with viscosity and pressure set to prevent capillary transit and venous filling. The lungs were fixed and scanned on a specimen computed tomography scanner at 8-μm resolution, and the vessels were segmented. Vessels were grouped into categories based on lumen diameter and branch generation. Results Robust high-resolution segmentation was achieved, permitting detailed quantitation of pulmonary vascular morphometrics. As expected, postnatal lung development was associated with progressive increase in small-vessel number and arterial branching complexity. Conclusions These methods for quantitative analysis of the pulmonary vasculature in postnatal and adult mice provide a useful tool for the evaluation of mouse models of disease that affect the pulmonary vasculature.

LanguageEnglish (US)
Pages115-122
Number of pages8
JournalJournal of Surgical Research
Volume207
DOIs
StatePublished - Jan 1 2017

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Tomography
Lung
Blood Vessels
X-Ray Computed Tomography Scanners
Pressure
Viscosity
Pulmonary Artery
Lung Diseases
Dilatation
Morbidity
Mortality

Keywords

  • Computed tomography
  • Lung development
  • Pulmonary circulation

ASJC Scopus subject areas

  • Surgery

Cite this

A method for evaluating the murine pulmonary vasculature using micro-computed tomography. / Phillips, Michael R.; Moore, Scott M.; Shah, Mansi; Lee, Clara; Lee, Yueh Z.; Faber, James E.; McLean, Sean E.

In: Journal of Surgical Research, Vol. 207, 01.01.2017, p. 115-122.

Research output: Contribution to journalArticle

Phillips MR, Moore SM, Shah M, Lee C, Lee YZ, Faber JE et al. A method for evaluating the murine pulmonary vasculature using micro-computed tomography. Journal of Surgical Research. 2017 Jan 1;207:115-122. Available from, DOI: 10.1016/j.jss.2016.08.074
Phillips, Michael R. ; Moore, Scott M. ; Shah, Mansi ; Lee, Clara ; Lee, Yueh Z. ; Faber, James E. ; McLean, Sean E./ A method for evaluating the murine pulmonary vasculature using micro-computed tomography. In: Journal of Surgical Research. 2017 ; Vol. 207. pp. 115-122
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