A KRAS GTPase K104Q mutant retains downstream signaling by offsetting defects in regulation

Guowei Yin, Samantha Kistler, Samuel D. George, Nora Kuhlmann, Leslie Garvey, Minh Huynh, Rachel K. Bagni, Michael Lammers, Channing J. Der, Sharon L. Campbell

Research output: Research - peer-reviewArticle

Abstract

The KRAS GTPase plays a critical role in the control of cellular growth. The activity of KRAS is regulated by guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and also post-translational modification. Lysine 104 in KRAS can be modified by ubiquitylation and acetylation, but the role of this residue in intrinsic KRAS function has not been well characterized. We find that lysine 104 is important for GEF recognition, because mutations at this position impaired GEF-mediated nucleotide exchange. Because the KRAS K104Q mutant has recently been employed as an acetylation mimetic, we conducted a series of studies to evaluate its in vitro and cell-based properties. Herein, we found that KRAS K104Q exhibited defects in both GEF-mediated exchange and GAP-mediated GTP hydrolysis, consistent with NMR-detected structural perturbations in localized regions of KRAS important for recognition of these regulatory proteins. Despite the partial defect in both GEF and GAP regulation, KRAS K104Q did not alter steady-state GTP-bound levels or the ability of the oncogenic KRAS G12V mutant to cause morphologic transformation of NIH 3T3 mouse fibroblasts and of WT KRAS to rescue the growth defect of mouse embryonic fibroblasts deficient in all Ras genes. We conclude that the KRAS K104Q mutant retains both WT and mutant KRAS function, probably due to offsetting defects in recognition of factors that up-regulate (GEF) and down-regulate (GAP) RAS activity.

LanguageEnglish (US)
Pages4446-4456
Number of pages11
JournalJournal of Biological Chemistry
Volume292
Issue number11
DOIs
StatePublished - Mar 17 2017

Fingerprint

Guanine Nucleotide Exchange Factors
GTP Phosphohydrolases
Defects
GTPase-Activating Proteins
Acetylation
Guanosine Triphosphate
Lysine
Fibroblasts
Growth
ras Genes
Ubiquitination
Post Translational Protein Processing
Hydrolysis
Up-Regulation
Down-Regulation
Nucleotides
Mutation
Proteins
In Vitro Techniques
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A KRAS GTPase K104Q mutant retains downstream signaling by offsetting defects in regulation. / Yin, Guowei; Kistler, Samantha; George, Samuel D.; Kuhlmann, Nora; Garvey, Leslie; Huynh, Minh; Bagni, Rachel K.; Lammers, Michael; Der, Channing J.; Campbell, Sharon L.

In: Journal of Biological Chemistry, Vol. 292, No. 11, 17.03.2017, p. 4446-4456.

Research output: Research - peer-reviewArticle

Yin, G, Kistler, S, George, SD, Kuhlmann, N, Garvey, L, Huynh, M, Bagni, RK, Lammers, M, Der, CJ & Campbell, SL 2017, 'A KRAS GTPase K104Q mutant retains downstream signaling by offsetting defects in regulation' Journal of Biological Chemistry, vol 292, no. 11, pp. 4446-4456. DOI: 10.1074/jbc.M116.762435
Yin G, Kistler S, George SD, Kuhlmann N, Garvey L, Huynh M et al. A KRAS GTPase K104Q mutant retains downstream signaling by offsetting defects in regulation. Journal of Biological Chemistry. 2017 Mar 17;292(11):4446-4456. Available from, DOI: 10.1074/jbc.M116.762435
Yin, Guowei ; Kistler, Samantha ; George, Samuel D. ; Kuhlmann, Nora ; Garvey, Leslie ; Huynh, Minh ; Bagni, Rachel K. ; Lammers, Michael ; Der, Channing J. ; Campbell, Sharon L./ A KRAS GTPase K104Q mutant retains downstream signaling by offsetting defects in regulation. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 11. pp. 4446-4456
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