A direct and selective electrochemical hydrogen sulfide sensor

Micah D. Brown, Jackson R. Hall, Mark H Schoenfisch

Research output: Contribution to journalArticle

Abstract

Continuous, in situ detection of hydrogen sulfide (H2S) in biological milieu is made possible with electrochemical methods, but direct amperometry is constrained by the generation of elemental sulfur as an oxidative byproduct. Deposition of a sulfur layer passivates the working electrode, reducing sensitivity and causing performance variability. Herein, we report on the use of a surface preconditioning procedure to deposit elemental sulfur on a glassy carbon electrode prior to measurement and evaluate performance with common analytical metrics. The lack of traditional anti-poisoning techniques (e.g. redox mediators, cleaning pulses) also allowed for facile surface modification with electropolymerized films. For the first time, a series of electropolymerized films were characterized for their H2S permselective behavior against common biological interferents. Highly selective, film-modified electrodes were then evaluated for their anti-biofouling ability in simulated wound fluid. The final optimized electrode was capable of measuring H2S with a low detection limit (i.e., <100 nM) and ∼80% of its initial sensitivity in proteinaceous media.

LanguageEnglish (US)
Pages67-76
Number of pages10
JournalAnalytica Chimica Acta
Volume1045
DOIs
StatePublished - Jan 3 2019

Fingerprint

Hydrogen Sulfide
hydrogen sulfide
Electrodes
electrode
Sulfur
sensor
Sensors
sulfur
Biofouling
electrochemical method
biofouling
Glassy carbon
poisoning
Poisoning
Oxidation-Reduction
Byproducts
Surface treatment
Limit of Detection
Cleaning
Carbon

Keywords

  • Anti-biofouling
  • Electropolymerized film
  • Hydrogen sulfide
  • Phenylenediamine
  • Sulfur poisoning
  • Surface conditioning

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

A direct and selective electrochemical hydrogen sulfide sensor. / Brown, Micah D.; Hall, Jackson R.; Schoenfisch, Mark H.

In: Analytica Chimica Acta, Vol. 1045, 03.01.2019, p. 67-76.

Research output: Contribution to journalArticle

Brown, Micah D. ; Hall, Jackson R. ; Schoenfisch, Mark H. / A direct and selective electrochemical hydrogen sulfide sensor. In: Analytica Chimica Acta. 2019 ; Vol. 1045. pp. 67-76.
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