[44] Reconstitution of Functional Sarcoplasmic Reticulum Membrane Vesicles

Gerhard Meissner, Sidney Fleischer

Research output: Contribution to journalArticle

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Abstract

This chapter describes the dissociation and reconstitution of functional sarcoplasmic reticulum vesicles, i.e., those that are again capable of energized Ca2+ uptake and loading. Purified sarcoplasmic reticulum vesicles are first disaggregated with deoxycholate and then reassembled by removal of the detergent using controlled dialysis. The reconstituted membrane vesicles have been reisolated and found to be of similar morphology, composition, and function to the original sarcoplasmic reticulum. This procedure can also be used with slight modification for preparing functional membrane vesicles composed mainly of phospholipid and the Ca2+ pump protein of sarcoplasmic reticulum.

LanguageEnglish (US)
Pages475-481
Number of pages7
JournalMethods in enzymology
Volume32
Issue numberC
DOIs
StatePublished - Jan 1 1974

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Sarcoplasmic Reticulum
Membranes
Deoxycholic Acid
Dialysis
Detergents
Phospholipids
Pumps
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

[44] Reconstitution of Functional Sarcoplasmic Reticulum Membrane Vesicles. / Meissner, Gerhard; Fleischer, Sidney.

In: Methods in enzymology, Vol. 32, No. C, 01.01.1974, p. 475-481.

Research output: Contribution to journalArticle

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