(19) F NMR spectroscopy as a probe of cytoplasmic viscosity and weak protein interactions in living cells

Yansheng Ye, Xiaoli Liu, Zeting Zhang, Qiong Wu, Bin Jiang, Ling Jiang, Xu Zhang, Maili Liu, Gary J. Pielak, Conggang Li

Research output: Contribution to journalArticle

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Abstract

Protein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein-protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging. Herein, we demonstrate the use of (19) F NMR spectroscopy to measure cytoplasmic viscosity and to characterize nonspecific protein-protein interactions in living Escherichia coli cells. The origins of resonance broadening in Escherichia coli cells were also investigated. We found that sample inhomogeneity has a negligible effect on resonance broadening, the cytoplasmic viscosity is only about 2-3 times that of water, and ubiquitous transient weak protein-protein interactions in the cytosol play a significant role in governing the detection of proteins by using in-cell NMR spectroscopy.

LanguageEnglish (US)
Pages12705-12710
Number of pages6
JournalChemistry (Weinheim an der Bergstrasse, Germany)
Volume19
Issue number38
DOIs
StatePublished - Sep 16 2013

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Nuclear magnetic resonance spectroscopy
Cells
Viscosity
Proteins
Escherichia coli
Water

Keywords

  • cytoplasmic viscosity
  • fluorine
  • NMR spectroscopy
  • transient weak interactions

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

(19) F NMR spectroscopy as a probe of cytoplasmic viscosity and weak protein interactions in living cells. / Ye, Yansheng; Liu, Xiaoli; Zhang, Zeting; Wu, Qiong; Jiang, Bin; Jiang, Ling; Zhang, Xu; Liu, Maili; Pielak, Gary J.; Li, Conggang.

In: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 19, No. 38, 16.09.2013, p. 12705-12710.

Research output: Contribution to journalArticle

Ye, Y, Liu, X, Zhang, Z, Wu, Q, Jiang, B, Jiang, L, Zhang, X, Liu, M, Pielak, GJ & Li, C 2013, '(19) F NMR spectroscopy as a probe of cytoplasmic viscosity and weak protein interactions in living cells' Chemistry (Weinheim an der Bergstrasse, Germany), vol. 19, no. 38, pp. 12705-12710. DOI: 10.1002/chem.201301657
Ye, Yansheng ; Liu, Xiaoli ; Zhang, Zeting ; Wu, Qiong ; Jiang, Bin ; Jiang, Ling ; Zhang, Xu ; Liu, Maili ; Pielak, Gary J. ; Li, Conggang. / (19) F NMR spectroscopy as a probe of cytoplasmic viscosity and weak protein interactions in living cells. In: Chemistry (Weinheim an der Bergstrasse, Germany). 2013 ; Vol. 19, No. 38. pp. 12705-12710
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