XL-I Analytical Ultracentrifuge and Fluorescence Optics

Project: Research project

Description

The UNC Macromolecular Interactions Facility, also called the'Mac-In-Fac'
(web address: macinfac.bio.unc.edu), is a core facility of the University
of North carolina at Chapel Hill. The facility has been operating since
1996. The facility has a Biacore X, a Biacore 2000, a Beckman Optima XL-A
Analytical Ultracentrifuge (AUC) with only scanning UV/VISIBLE optics, a
fluorescence spectrophotometer, differential and isothermal scanning
calorimeters, circular dichroism spectropolarimeters, and static and
dynamic light scattering instruments.There are no similar facilities on
the UNC Chapel Hill Campus, or nearby institutions. Its current users come
from 11 departments on the UNC Chapel Hill campus (Biochemistry &
Biophysics, Biology, Cell Biology, Chemistry, Microbiology & Immunology,
Pathology, Pediactrics, Pharmacology, Pharmacy, Physics, and Physiology),
other academic and research institutions from North Carolina and several
Research Triangle Park biotechnology companies.

Many of our users have experienced problems while carrying out their
projects using the absorbance optics available with the Optima XL-A AUC.
These and other projects need to be carried out with the interference
optics available with the Optima XL-I AUC (please see attached specific
research plans). No such instrument is available at neighboring
universities (Duke and NC State). Additionally, some other users have a
need for an AUC with a fluorescence optics,based detection system (please
see attached specific research plans) Therefore, the Mac-In-Fac requests
funds for:

(1)purchasing an interference and absorbance optics based AUC (Beckman
Optima XL-I,and

(2) for modifying our existing Optima XL-A AUC with a fluorescence optical
detection system (the modification will be carried out by the Center to
Advance Molecular Interaction Sciences, University of New Hampshire).

The requested instrument and accessary will be used by many NIH-supported
investigators as well as investigators supported by other fonding agencies
with a total usage of more than 75%.
StatusFinished
Effective start/end date4/1/033/31/05

Funding

  • NIH National Center for Research Resources (NCRR)

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Optics
Fluorescence
Cytology
Immunology
Biophysics
Microbiology
Biochemistry
Molecular interactions
Spectrophotometers
Physiology
Pathology
Purchasing
Biotechnology
Light scattering
Physics
Scanning
Industry